Human papillomavirus 52 positive squamous cell carcinoma of the conjunctiva

Hpv and p16, Autentificare

In addition to tobacco and alcohol abuse, certain viruses have been associated with squamous cell carcinoma SCC of the head and neck, causing alterations in DNA.

Department of Ophthalmology, Grigore T. E-mail: moc. We report the detection of HPV 52 in a sample taken from a year-old patient with squamous cell carcinoma of the conjunctiva of the left eye. The method used for the detection of HPV was real time polymerase chain reaction.

It has been demonstrated that the human papil­loma­virus HPV type 16, a subtype of the human pa­pil­loma­virus, is present in the oropharyngeal carcinomas of non-smokers patients inclusive. HPV-infected cells express some viral proteins encoded by genes called E6 and E7, and can inactivate p53 protein and the retinoblastoma-type pro­tein RBP involved in the regulation of proliferation and cell death.

Materials and method.

Human papillomavirus 52 positive squamous cell carcinoma of the conjunctiva

We present an immunohistochemical study conducted to identify significant tumour markers in tonsillar SCC. We present the sta­tis­tically significant correlations between the presence of immunohistochemical markers and studied local re­cur­rence, lymph node recurrence and risk of a second can­cer in the aerodigestive upper tract.

Acest test identifica cu inalta sensibilitate si specificitate riscul de hpv and p16 catre leziuni cervicale intraepiteliale de grad inalt. Cancerul de col uterin este a treia cauza de cancer la femei si este determinat in principal de infectia cu virusul HPV Human Papilloma virustulpini cu risc crescut.

The de­mon­stration of HPV in tonsillar tumour tissue requires in situ hybridization or polymerase chain reaction PCR for the evidence of viral genome included into the host cell. The practical implications of an etiologic role of HPV in head hpv and p16 neck cancer generally and in tonsillar SCC in particular remains in question and is in relate with prog­nosis, treatment and prevention.

În ciuda contribuției recunoscute a citologiei la scăderea substanțială a cancerului de col uterin în țările cu program organizat de screening pentru această boală, este bine cunoscut astăzi faptul că depistarea leziunilor precanceroase prin citologia Hpv and p16 are o sensibilitate limitată, ea asociindu-se cu un număr inevitabil de rezultate fals negative. Pe de altă parte, demonstrarea relației de cauzalitate între infecția HPV și cancerul de col a condus la dezvoltarea unor noi teste de biologie moleculară, între care cele mai importante sunt testele pentru ADN HPV, care au o sensibilitate foarte înaltă. Dar rezultatul acestor teste este relevant clinic doar atunci când este negativ, situație în hpv and p16 el oferă o garanție puternică asupra riscului foarte scăzut al pacientei pentru cancer de col uterin în următorii ani și permite mărirea intervalelor de screening. Datorită caracterului infecției HPV, care este în același timp foarte răspândită și în marea majoritate a cazurilor spontan trecătoare, testul pentru HPV, deși foarte sensibil, are o specificitate limitată pentru hpv and p16 și cancer, el asociindu-se cu un număr inevitabil de rezultate fals pozitive. Din aceste motive, combinația între citologie și testarea HPV, efectuate fie simultan, fie succesiv, este o modalitate de compensare reciprocă între sensibilitatea și specificitatea fiecăruia dintre cele două teste și reprezintă în prezent strategia logică pe care se construiesc programele de prevenție.

În afară de hpv and p16 de tutun şi abuzul de al­cool, anumite virusuri au fost asociate cu carcinomul cu celule scuamoase CCS al capului şi gâtului, cauzând al­te­rări la nivelul ADN-ului. Este dovedit că virusul papiloma uman HPVtipul 16, este prezent la nivelul carcinoamelor orofaringiene inclusiv în cazul nefumătorilor.

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Celulele in­fec­ta­te cu HPV exprimă unele proteine virale codate de ge­ne­le denumite E6 şi E7 şi pot inactiva proteina p53 şi pro­tei­na de tip retinoblastom RBP implicate în reglarea pro­li­fe­ră­rii şi morţii celulare. Materiale şi metodă. Pre­zen­tăm un stu­diu imunohistochimic realizat cu scopul de a identifica mar­keri tumorali semnificativi în CCS de amig­da­lă.

Pre­zen­tăm co­re­la­ţiile semnificative statistic între prezenţa mar­ke­rilor imu­no­his­to­chimici şi recurenţa locală, recurenţa no­du­lilor limfatici şi ris­cul apariţiei unui al doilea cancer hpv and p16 trac­tul aerodigestiv su­pe­rior.

Autentificare

Punerea în evidenţă a HPV-ului în ţesutul tu­mo­ral amigdalian necesită hibridizare in situ şi reacţie de polimerizare în lanţ PCR pentru punerea în evidenţă a genomului viral conţinut în celula-gazdă. Im­pli­caţiile practice ale unui rol etiologic al HPV-ului în can­ce­rele de cap şi gât, în general, şi în CCS de hpv and p16, în par­ti­cu­lar, reprezintă un subiect în dezbatere, fiind în relaţie cu prog­nos­ticul, cancer abdominal lining şi prevenţia acestor tipuri de can­cere.

Cuvinte cheie carcinomul cu celule scuamoase de amigdală CCS HPV markeri tumorali Hpv and p16 The tonsillar squamous hpv and p16 carcinoma SCC is becoming a public health problem because of its rising incidence in the last 20 years, in contrast to the decreasing incidence of carcinomas in other subsites of head and neck associated to the reduced prevalence of smoking.

These tumours hpv and p16 oral cavity, oropha­rynx, larynx, hypopharynx and sinonasal region are linked by common characteristics, including a male predominant hpv and p16 in the 5th-6th decade of life, an important etiological link with tobacco, alcohol use or betel nut chewing, and a histopathological resemblance 1.

Human papillomavirus 52 positive squamous cell carcinoma of the conjunctiva

Data regarding the epidemiology revealed that in Romania hpv and p16 oropharyngeal cancer represents 2. In France, during the last 30 years, the mortality in papilloma of sinus and oropharyngeal cancer increased by three times 1.

As in cervical cancers, the oropharyngeal infection with HPV is intraductal papilloma emed sexually transmitted disease which involves some particularities of sexual behaviour: a large number of vaginal sex partners, oral and anal sex.

The recent increasing of OPSCC incidence may reflect the social changes regarding sexual behaviour in the modern world 6. The anatomical sites preferred by HPV in oropharynx are the tonsils and the hpv and p16, because of the unique presence of transitional mucosa in oropharynx and particular in tonsillar tissue, which presents important histological similarities with the cervical hpv and p16.

Tonsillar epithelium invagination may favour virus capture and promote its access to basal cells the only dividing cells in the epithelium. The tonsillar tissue could be a reservoir for HPV in the upper aero digestive tract.

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We had two premises for our study on tonsillar cancers. The second consists in the fact that mutagens such as tobacco, alcohol and HPV viral oncogenes E6 and E7 induce dysfunctions of two major mechanisms of cellular cycle, which involves the p53 and RBP tumoral suppressor genes 2.

Materials and method We made an immunohistochemical retrospective study between andaiming to identify any correlations between tumoral markers and the evolution and prognosis in tonsillar SCC. Materials We studied 52 cases of patients diagnosed with tonsillar SCC.

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We had a first group Group I with 25 cases, where the positive diagnose was made by biopsy and these patients had radiotherapy as first curative method of treatment. We had a second group Group II with 27 cases, where the positive diagnose was made on surgical specimens and these patients had surgery as the first curative method of treatment. The two groups were similar regarding age and gender distribution.

The dilutions and markers specifications are revealed in Table 1. We also studied lymphocyte populations CD4, CD8, and populations of dendritic cells in tumour tissue.

Table 1. The dilutions and markers specifications For the immunohistochemical identification of tumoral antigens we used the three-stadial indirect method Avidine-Biotine-Peroxidase ABPafter Hsu and colab. Results The gender repartition of cases was: 47 male cases and 5 female cases.

Dictionar de analize

The age repartition of cases was: two cases between years old, 14 cases between years old, 21 cases between years old, 10 cases between years old, and five cases between years old. The correlation coefficient between the two sets of data, corresponding to Group I and Group II, was 0.

In both groups, we had 48 smoker patients, representing The patients who were both smokers and alcohol consumers represented We studied the tumoral markers on 52 cases of squamous cell carcinoma.

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Thirty-eight cases were well differentiated carcinoma and 14 cases were medium differentiated carcinoma. We present the results, that we considered immunohistochemically valid and statistically significant Table 2.

Case Report

Table 2. The distribution of tumoral markers in specimens of SCC studied We realised a correlation between the presence of the tumoral marker of a certain type positive and slowly positive results and the post-therapeutic evolution — local recurrence, nodal relapse, the occurrence of second cancers in upper aerodigestive upper ways and distance metastases.

We have had patients who had more than one recurrence in the same time. Our purpose was to identify the correlations between markers of evolution and prognosis in tonsillar SCC.

Our results indicate p53 protein and Hpv and p16 protein as tumoral markers of unfavourable prognosis hpv and p16 post-therapeutic evolution in tonsillar SCC. For TGFa, we can make a correlation between its level in tumoral tissue and the risk of loco-regional relapse.

For the HPV identification in tumoral tissue, we used the identification of capsid p16 protein, so we cannot make definitive conclusions referring at the presence or absence of HPV in the tumoral tissue for patients with tonsillar SCC.

But we realised a correlation between the presence of HPV and the type of post-therapeutic evolution Figures Figure 1. The presence of RBP protein hpv and p16 positive and slowly positive cases was associated with local recurrence in 29 cases The presence of TGF protein 41 positive and slowly positive cases was associated with local recurrence in 18 cases The presence of HPV capsid protein 14 positive cases was associated with local recurrence in nine cases Figure 6.

Tumoral markers in evolution of tonsillar SCC result of our retrospective study From our data, we can certify as prognostic factors in tonsillar SCC: T stage, N stage, performing or not an elective type of clinical negative neck N0, type of neck dissection, the total hpv and p16 of radiotherapy.

  1. Октопаук ответил ярко-алой вспышкой, за ней последовала бледно-зеленая полоса, затем лавандовая, две желтые разного оттенка (одна шафранная, другая лимонная с переходом в зеленый), и наконец все завершилось пурпурной полосой.

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  3. Николь долго не могла уснуть.

  4. Они прислушались.

  5. "Но как же могла исчезнуть подобная пара.